Sun. May 29th, 2022
All About Fluorescence Cuvettes, Polarization, and Spectrophotometers

A spectrophotometer (also called light spectrometer or optical spectrometer) is an instrument used to measure the properties of light over a specific wavelength range. A good spectrophotometer can be used to measure samples of different compositions, determine the concentration of substances in solution, and much more. CotsLab supplies full sets of spectroscopy equipment, including some of the most popular instruments and accessories for measuring fluorescence cuvettes intensity and polarization, including various cuvettes.

The Importance of Measuring Transmittance

Transmittance is measured by placing a cuvette in front of a light source. Typically, a spectrophotometer can report transmittance at any wavelength within its range. Measuring transmittance provides valuable information about your sample, such as: Transmittance correlates directly with absorbance; thus, measuring transmittance is an effective way to calibrate a spectrophotometer using an absorbance standard. As such, many spectrophotometers are designed to measure both absorbance and transmittance. If you’re lucky enough to have such a machine, then it’s certainly preferable to use it! If not – don’t worry – we’ll discuss several ways you can do it yourself with just materials from around your lab.

What Is a CotsLab Spectrophotometer?

To understand what a CotsLab spectrophotometer is, it’s important to know what a spectrophotometer is. A spectrophotometer is a scientific instrument that measures either absorbance or transmittance. In other words, it tells you how much light energy passes through or gets absorbed by your sample as a function of wavelength. Spectrometers are used in all sorts of industries for applications like controlling color uniformity (CMYK), solving crimes with fingerprint powder (forensics), testing cancer cells for enzymes secreted by tumors (biomedicine), detecting concentrations of organics in drinking water (environmental), etc.

Five Types of Cuvettes Used in Spectroscopy

There are several types of cuvettes used in spectroscopy. The four commonly used cuvettes include round bottom (RB), flat bottom (FB), modified rectangle (MR) and rectangular (RT). These cuvettes are specifically designed for UV-Vis spectroscopy applications to capture changes in light as a function of wavelength. Understanding these differences will help you choose an appropriate type of cuvette for your application. A round bottom or RB cell has a flat base with a spherical well with a uniform cross-section and its height. The angle between the flat surface and well wall is 60 degrees, with 90-degree walls at both ends of the well.

Why Choose CotsLab Fluorescence Polarization Cuvettes?

Over 150 years ago, chemists discovered that a particular light source could excite fluorescence more strongly than another light source. That discovery enabled chemists to learn how pigments absorb or scatter different wavelengths of light. For example, it helped them to distinguish between hydrogen peroxide (toxic) and carbon dioxide (inert). It also enabled them to identify areas where contamination had occurred in samples during chemical analysis. Although researchers had previously developed analytical tools for measuring emissions from excited states of atoms and molecules – known as photoluminescence spectroscopy – no tools existed for measurements made using fluorescence techniques.

Why Choose CotsLab Fluorescence Lifetime Cuvettes?

A lifetime measurement is a measurement technique used to measure fluorescence decay kinetics. It is based on fluorophore’s lifetime (instead of intensity decay), making it possible to observe fluorescence. This technique allows the determination of different kinetic parameters from one single experiment: first-order rate constant k_1, association rate constant k_assoc, disassociation rate constant k_dis, the decay time tau or fluorescent lifetimes tau_Lambda etc.

CotsLab offers dyes for all five types of cuvettes

UVA, polychromatic visible (PCV), polarized light (PL), fluorescence lifetime (FL), and fluorescence polarization. CotsLab offers a range of quality dyes for all your needs. For example, you can use our polychrome dyes in both PCV and PL cuvettes to measure absorbance. You can then choose from several different FL dyes to determine fluorescence lifetime by using cuvettes with a fixed wavelength filter or opt for our spectral dye that measures both fluorescence intensity and lifetime measurements simultaneously. When it comes to spectrometers, they offer two dye sets with various spectral properties so that you will be able to choose between an emission slit-scan or peak scan setting on your device.

Bottom Line

Thanks for joining us! Please visit their website for more information on how you can use CotsLab products to improve your research workflows. If you have any questions or comments about fluorescence spectroscopy or fluorescence cuvettes, please leave them in a comment below.

We look forward to hearing from you!

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